ProSci

Nebulette Antibody

Product Code:
 
PSI-42-163
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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CodeSizePrice
PSI-42-163-0.1mg0.1mg£505.00
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Further Information

Additional Names:
NEBL, nebulette, FLJ53769, LNEBL, MGC119746, MGC119747, bA56H7.1, LIM-nebulette, NEBL, actin-binding Z-disc protein, OTTHUMP00000019268, actin-binding Z-disk protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1ug/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Background References:
  • Bonzo JR, Norris AA, Esham M, Moncman CL, The nebulette repeat domain is necessary for proper maintenance of tropomyosin with the cardiac sarcomere. Exp Cell Res. 2008 Nov 15;314(19):3519-30. Epub 2008 Sep 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-ELQRLKRTQE
NCBI Gene ID #:
10529
NCBI Official Name:
nebulette
NCBI Official Symbol:
NEBL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_998734.1, NP_001166955.1
Protein GI Number:
47087157
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
Swissprot #:
O76041