ProSci

SNX26 Antibody

Product Code:
 
PSI-46-402
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-402 (2.5ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-402 (2.5ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-402-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
SNX26, TCGAP, FLJ39019, sorting nexin 26, likely ortholog of mouse TC10/CDC42 GTPase-activating protein, NOMA-GAP, neurite outgrowth multiadaptor RhoGAP protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Bone Marrow, Kidney, and Pancreas lysates after 1ug/ml antibody staining. EB05916 may detect the isoform A1A5D2 (UniProtKB) with a molecular weight of 81.4kDa which is similar in amino acid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently we cannot find a precise explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Brain shows cytoplasm staining of Purkinje cells in Cerebellum. Recommended concentration, 2.5ug/ml.
Background References:
  • Teasdale RD, Loci D, Houghton F, Karlsson L, Gleeson PA. A large family of endosome-localized proteins related to sorting nexin 1. Biochem J. 2001 Aug 15;358(Pt 1):7-16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: SWSLHSEGQTRSYC
NCBI Gene ID #:
115703
NCBI Official Name:
Rho GTPase activating protein 33
NCBI Official Symbol:
SNX26
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_443180.2
Protein GI Number:
23510413
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O14559