ProSci

IL-1B Antibody

Product Code:
 
PSI-XP-5179Bt
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
IL-1B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
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To detect mIL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine IL-1-beta (XP-5179) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant mIL-1-beta.
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To detect mIL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine IL-1-beta (XP-5179) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant mIL-1-beta.

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CodeSizePrice
PSI-XP-5179Bt-0.05mg0.05mg£524.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
Il-1b, IL-1betaInterleukin-1 betaIL-1 beta
Application Note:
ELISA:

Sandwich:
To detect mIL-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-1β (XP-5179) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1β.

Western Blot:

To detect mIL-1- β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1- β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1- β (murine Interleukin-1- β).
NCBI Gene ID #:
16176
NCBI Official Name:
interleukin 1 beta
NCBI Official Symbol:
Il1b
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P10749
Protein GI Number:
124304
Purification:
Anti-mIL-1- β specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P10749
User NOte:
Centrifuge vial prior to opening.