Further Information
SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88
WB: 1 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot, Immunohistochemistry, Immunofluorescence in human samples. All other applications and species not yet tested.
The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1). SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3). Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1).
- Rice et al. Nat. Genet. 2009; 41:829-32.
- Hrecka et al. Nature 2011; 474:654-7.
- Welbourn et al. J. Virol. 2013; 87:11516-24.
SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Anti-SAMHD1 antibody (8007) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human SAMHD1.
Human SAMHD1 has 3 isoforms, including isoform 1 (626aa, 72kD), isoform 3 (556aa, 64kD), isoform 4 (591aa, 68kD). Mouse SAMHD1 has 2 isoforms, including (658aa, 76kD) and (651aa, 75kD). Rat SAMHD1 has only one isoform (620aa, 72kD). 8007 can detect human, mouse and rat.
25939
SAM domain and HD domain 1
SAMHD1
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 72kD
Observed: 72 kD
NP_056289
38016914
SAMHD1 antibody is affinity chromatography purified via peptide column.
Innate Immunity
SAMHD1 antibody is human and mouse reactive.
Q9Y3Z3
Optimal dilutions for each application to be determined by the researcher.
KO Validation (Figure 5) shows SAMHD1 expression detected by anti-SAMHD1 antibodies (8007) was disrupted in multiple cells of SAMHD1 KO mice (figure 6).
Overexpression validation (Figure 2,6,7): SAMHD1 overexpression detected by anit-SAMHD1 antibodies (8007) was observed in normal cell lines stably across all healthy donors.
Regulated expression validation (Figure 8 & 9): SAMHD1 expression detected by anit-SAMHD1 antibodies (8007) was down-regulated by nucleofection with miR-181b at 48hr (figure 8), but up-regulated by treatment of miR-181family inhibitor (figure 9).