Further Information
SREBF1 Antibody: SREBP1, bHLHd1, SREBP-1c, BHLHD1, SREBP1, Sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, SREBP-1
SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4).
- Wang X, Briggs MR, Hua X, et al. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization. J. Biol. Chem. 1993; 268:14497-504.
- Hua X, Yokoyama C, Wu J, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA 1993; 90:11603-7.
- Wang X, Sato R, Brown MS, et al. SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell 1994; 77:53-62.
- Raghow R, Yellaturu C, Deng X, et al. SREBPs: the crossroads of physiological and pathological homeostasis. Endocrinol. Metab. 2008; 19:65-73.
SREBF1 antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
SREBF1 antibody was raised against a 17 amino acid peptide near the center of human SREBF1.
The immunogen is located within amino acids 450 - 500 of SREBF1.
6720
sterol regulatory element binding transcription factor 1
SREBF1
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 129 kDa
Observed: 132 kDa
NP_001005291
52630419
SREBF1 antibody is affinity chromatography purified via peptide column.
Obesity
SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2.
P36956
Optimal dilutions for each application to be determined by the researcher.