Further Information
Sex hormone-binding globulin, ABP, SBP, TEBG
SHBG antibody can be used for detection of SHBG by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SHBG is a steroid binding protein that was first described as a plasma protein secreted by the liver and is thought to participate in the regulation of steroid responses. SHBG transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers (1). Low plasma SHBG levels are associated with obesity, abdominal adiposity, metabolic syndrome, and predict the development of type 2 diabetes (2,3). Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus (4).
- Siiteri PK, Murai JT, Hammond GL, et al. The serum transport of steroid hormones. Recent Prog. Horm. Res. 1982; 38:457-510.
- Li C, Ford ES, Li B, et al. Association of testosterone and sex hormone-binding globulin with metabolic syndrome and insulin resistance in men. Diabetes Care 2010; 33:1618-24.
- Ding EL, Song Y, Manson JE, et al. Sex hormone-binding globulin and risk of type 2 diabetes in women and men. N. Engl. J. Med. 2009; 361:1152-63.
- Hacihanefioglu B, Aybey B, Hakan Ozon Y, et al. Association of anthropometric, androgenic and insulin-related features with polymorphisms in exon 8 of SHBG gene in women with polycystic ovary syndrome. Gynecol. Endocrinol. 2013; 29:361-4.
SHBG antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Rabbit: (75%)
SHBG antibody was raised against a 16 amino acid peptide near the center of human SHBG.
The immunogen is located within amino acids 220 - 270 of SHBG.
6462
sex hormone-binding globulin
SHBG
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 25, 26, 31, 32, 38, 44 kDa
Observed: 35 kDa
NP_001031
7382460
SHBG antibody is affinity chromatography purified via peptide column.
Obesity
SHBG antibody is human, mouse, and rat reactive. At least three isoforms of SHBG are known to exist; this antibody will detect all three isoforms.
P04278
Optimal dilutions for each application to be determined by the researcher.