Further Information
CMD1W, CMH15, Metavinculin, MVCL
Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
- Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
- Gilmore et al. J. Cell Sci. 1992; 103:719-31.
- Deakin et al. PLoS One 2012; 7:e37990.
- Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Pig: (100%)
Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
The immunogen is located within amino acids 50 - 100 of Vinculin.
7414
vinculin
VCL
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 117 kDa
Observed: 120 kDa
NP_003364
4507877
Vinculin antibody is purified via Protein A prification.
Homeostasis
Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Optimal dilutions for each application to be determined by the researcher.