SAMHD1 (phospho Thr592) Antibody
PSI-8005
Rabbit
IgG
Polyclonal
RUO
- Human
- Mouse
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
SAMHD1 (phospho Thr592) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
PSI-8005-0.02mg | 0.02mg | £150.00 |
PSI-8005-0.1mg | 0.1mg | £449.00 |
* Rare exceptions are clearly labelled (only 0.14% of items!).
Typical lead time: 14-21 working days.
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Further Information
Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
- Rice et al. Nat. Genet. 2009; 41:829-32.
- Hrecka et al. Nature 2011; 474:654-7.
- Welbourn et al. J. Virol. 2013; 87:11516-24.
Observed: 72 kD
Mutant validation (Figure 2, 5, 6 & 7): Anti-SAMHD1 (phospho Thr592) antibody (8005) specificity was further verified by SAMHD1 (phospho Thr592) mutant. SAMHD1 (phospho Thr592) signal in 293T cells (figure 2) or HEK293T cells (figure 5 & 7) or U937 cells (figure 6) transfected with SAMHD1 (phospho Thr592) mutant was disrupted in comparison with that in cells transfected with the wild-type.
KD validation (Figure 8): SAMHD1 (phospho Thr592) expression detected by anit-SAMHD1 (phospho Thr592) antibodies (8005) was disrupted in knockdown macrophages.
Regulated expression validation (Figure 10): SAMHD1 (phospho Thr592) expression detected by anit-SAMHD1 (phospho Thr592) antibodies (8005) was down-regulated by lambda-phosphatase treatment in S and G2 phase of cell cycle.
Documents
References
- Antonucci et al. SAMHD1-mediated HIV-1 restriction in cells does not involvePMID: 27711056
- Kueck et al. The aryl hydrocarbon receptor and interferon gamma generate antiviral states via transcriptional repression. Elife. 2018;7. PMID: 30132758
- St Gelais et al. A Cyclin-Binding Motif in Human SAMHD1 Is Required for Its HIV-1 Restriction, dNTPase Activity, Tetramer Formation, and Efficient Phosphorylation. J Virol. 2018;92(6)PMID: 29321329
- Tramentozzi et al. The dNTP triphosphohydrolase activity of SAMHD1 persists during S-phase when the enzyme is phosphorylated at T592. Cell Cycle. 2018;17(9):1102-1114. PMID: 30039733
- Mlcochova et al. A G1-like state allows HIV-1 to bypass SAMHD1 restriction in macrophages. EMBO J. 2017;36(5):604-616.PMID: 28122869
- Mlcochova et al. DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages. EMBO J. 2018;37(1):50-62. PMID: 29084722
- Herrmann et al. The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation. Mob DNA. 2018;9:11. PMID: 29610582
- Osei et al. USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1. J Virol. 2018;92(20). PMID: 30068654
- St Gelais et al. A Putative Cyclin-binding Motif in Human SAMHD1 Contributes to Protein Phosphorylation, Localization, and Stability. J Biol Chem. 2016;291(51):26332-26342. PMID: 27815502
- Wittmann et al. Phosphorylation of murine SAMHD1 regulates its antiretroviral activity. Retrovirology. 2015;12:103.PMID: 26667483
- Feifei Wang. Comparison of Murine and Human SAMHD1?s Role in Retroviral Restriction and Cell Cycle Progression. PhD Thesis. The Ohio State University. 2015.
- Alexandra Herrmann. The role of SAMHD1 in restriction and immune sensing of retroviruses and retroelements. PhD Thesis. The Friedrich Alexander University Erlangen-Nuremberg. 2018.