AdipoGen Life Sciences

Ac-Arg-Leu-Arg-AMC

Product Code:
 
AG-CP3-0013
Product Group:
 
Inhibitors and Activators
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
-20°C
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Chemical Structure
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Chemical Structure

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AG-CP3-0013-M0011 mg£55.00
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AG-CP3-0013-M0055 mg£200.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
Ac-RLR-AMC . TFA; Proteasome Substrate
Appearance:
Solid lyophilized powder.
CAS:
929903-87-7 (free base)
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Avoid freeze/thaw cycles.Protect from light.
InChi:
InChI=1S/C30H46N10O6.C2HF3O2/c1-16(2)13-23(40-27(44)21(37-18(4)41)7-5-11-35-29(31)32)28(45)39-22(8-6-12-36-30(33)34)26(43)38-19-9-10-20-17(3)14-25(42)46-24(20)15-19;3-2(4,5)1(6)7/h9-10,14-16,21-23H,5-8,11-13H2,1-4H3,(H,37,41)(H,38,43)(H,39,45)(H,40,44)(H4,31,32,35)(H4,33,34,36);(H,6,7)/t21-,22-,23-;/m0./s1
InChiKey:
CEEMPRHZPRGAAK-RGRVRPFLSA-N
Long Description:
Chemical. CAS: 929903-87-7 (free base). Formula: C30H46N10O6 . C2HF3O2. MW: 642.8 . 114.0. Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. Excitation: 380nm. Emission: 460nm. This substrate is useful for inhibitor screening and kinetic analysis.
MDL:
MFCD18782518
Molecular Formula:
C30H46N10O6 . C2HF3O2
Molecular Weight:
642.8 . 114.0
Other data:
Use: Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 µM. Activity with purified proteasome: Km=78µM.
Package Type:
Vial
Product Description:
Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. Excitation: 380nm. Emission: 460nm. This substrate is useful for inhibitor screening and kinetic analysis.
Purity:
>97% (HPLC)
Sequence:
Acetyl-Arg-Leu-Arg-7-amido-4-methylcoumarin
SMILES:
CC(C)C[C@H](NC(=O)[C@H](CCCNC(N)=N)NC(C)=O)C(=O)NC(CCCNC(N)=N)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2
Solubility Chemicals:
Soluble in 5% acetic acid.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Assessment of proteasome activity in cell lysates and tissue homogenates using peptide substrates: K.J. Rodgers & R.T. Dean; Int. J. Biochem. Cell Biol. 35, 716 (2003) | Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates: A.F. Kisselev & A.L. Goldberg; Methods Enzymol. 398, 364 (2005) | Importance of the different proteolytic sites of the proteasome and the efficacy of inhibitors varies with the protein substrate: A.F. Kisselev, et al.; J. Biol. Chem. 281, 8582 (2006) | 5,6,3',4'-Tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor: T.L. Chang, et al.; Planta Med. 76, 987 (2010)