Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
UBD antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Ubiquitin D, Diubiquitin, FAT10, GABBR1, UBD-3, Ubiquitin-like protein FAT10
UBD antibody can be used for detection of UBD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
UBD, also designated ubiquitin D or diubiquitin, plays a role in antigen presentation, cytokine response and apoptosis. It is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin (1,2). UBD is inducible by interferon gamma and TNF-alpha and regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha (3). It may also regulate cell growth during dendritic cell or B cell activation and development (4,5).
- Ocklenburg F, Moharregh-Khiabani D, Geffers R, et al. UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells. Lab. Invest. 2006; 86:724-37.
- Cook WJ, Jeffrey LC, Carson M, et al. Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2). J. Biol. Chem. 1992; 267:16467-71.
- Rahighi S, Ikeda F, Kawasaki M, et al. Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation. Cell 2009;136:1098-109.
- Cort L, Habib M, Eberwine RA, et al. Diubiquitin (Ubd) is a susceptibility gene for virus-triggered autoimmune diabetes in rats. Genes Immun. 2014; 15:168-75.
UBD antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Rat: (81%)
UBD antibody was raised against a 16 amino acid peptide near the center of human UBD.
The immunogen is located within amino acids 80 - 130 of UBD.
10537
Ubiquitin D
UBD
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 18 kDa
Observed: 17 kDa
NP_006389
222352096
UBD antibody is affinity chromatography purified via peptide column.
Apoptosis,Immunology
UBD antibody is human specific.
O15205
Optimal dilutions for each application to be determined by the researcher.