ProSci

SLC29A1 Antibody

Product Code:
 
PSI-8125
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
SLC29A1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
1 / 3
Western blot analysis of SLC29A1 in human ovary tissue lysate with SLC29A1 antibody at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of SLC29A1 in human ovary tissue with SLC29A1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of SLC29A1 in human ovary tissue with SLC29A1 antibody at 20 μg/mL.
❮❯

Western blot analysis of SLC29A1 in human ovary tissue lysate with SLC29A1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of SLC29A1 in human ovary tissue with SLC29A1 antibody at 5 μg/mL.
Immunofluorescence of SLC29A1 in human ovary tissue with SLC29A1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-8125-0.02mg0.02mg£150.00
Quantity:
PSI-8125-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Solute carrier family 29 member 1, Equilibrative nucleoside transporter 1, ENT1
Application Note:
SLC29A1 antibody can be used for detection of SLC29A1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SLC29A1 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A1 is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium (3). As a nucleoside transporter, SLC29A1 plays an important role in the uptake of nucleoside-based anti-cancer drugs; polymorphisms of point mutations in the gene encoding this protein may affect the efficacy of these drugs (4).
Background References:
  • Griffiths M, Beaumont N, Yao SY, et al. Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nat. Med. 1997; 3:89-93.
  • Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
  • Mangravite LM, Xiao G, and Giacomini KM. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am. J. Physiol. Renal Physiol. 284:F902-10.
  • Zimmerman EI, Huang M, Leisewitz AV, et al. Identification of a novel point mutation in ENT1 that confers resistance to Ara-C in human T cell leukemia CCRF-CEM cells. FEBS Lett. 2009; 583:425-9.
Buffer:
SLC29A1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (81%), Mouse: (75%)
Immunogen:
SLC29A1 antibody was raised against a 17 amino acid peptide near the center of human SLC29A1.

The immunogen is located within amino acids 240 - 290 of SLC29A1.
NCBI Gene ID #:
2030
NCBI Official Name:
solute carrier family 29 (equilibrative nucleoside transporter), member 1
NCBI Official Symbol:
SLC29A1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50 kDa

Observed: 53 kDa
Protein Accession #:
NP_004946
Protein GI Number:
4826716
Purification:
SLC29A1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Homeostasis
SPECIFICITY:
SLC29A1 antibody is human specific. SLC29A1 antibody is predicted to not cross-react with other SLC29 proteins.
Swissprot #:
Q99808
User NOte:
Optimal dilutions for each application to be determined by the researcher.