C-RAF (phospho Thr269) Antibody
Antibody Clonality:
Polyclonal
Application:
Immunohistochemistry (IHC)
Storage:
Store antibody at -20°C for up to one year.
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United States.
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Further Information
C-RAF, C-Raf, CRAF, RAF-1
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
- The MGC Project Team; Genome Res. 14:2121-2127(2004).
- Olsen J.V., Cell 127:635-648(2006).
- Mott H.R., Proc. Natl. Acad. Sci. U.S.A. 93:8312-8317(1996).
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
C-RAF (phospho Thr269) antibody was raised against a peptide sequence around phosphorylation site of threonine269 (S-T-T (p)-L-P) derived from Human C-RAF.
Phospho-Specific
5894
v-raf-1 murine leukemia viral oncogene homolog 1
RAF1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
NP_002871.1
4506401
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Phospho-Specific
The antibody detects endogenous levels of C-RAF only when phosphorylated at threonine 269.
P04049