ProSci

NF-κB p100 (phospho Ser872) Antibody

Product Code:
 
PSI-79-971
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20°C for up to one year.
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Western blot analysis of extracts from RAW264.7 cells treated with EGF using Phospho-NF-κB p100 (Ser872) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from RAW264.7 cells treated with EGF using Phospho-NF-κB p100 (Ser872) Antibody. The lane on the right is treated with the antigen-specific peptide.

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PSI-79-971-0.1mg0.1mg£488.00
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Further Information

Additional Names:
DNA-binding factor KBF2, Lymphocyte translocation chromosome 10, Lyt10, Nuclear factor NF-kappa-B p100 subunit, Oncogene Lyt-10
Application Note:
Western Blot: 1:500~1:1000
Background:
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.
Background References:
  • Qu Z, Qing G, Rabson A, Xiao G (2004) J Biol Chem 279, 44563-72 . Xiao G, Fong A, Sun SC (2004) J Biol Chem 279, 30099-105 .
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NF-κB p100 (phospho Ser872) antibody was raised against a peptide sequence around phosphorylation site of serine872 (S-Q-S (p)-V-E) derived from Human NF-κB p100 .
Modifications:
Phospho-Specific
NCBI Gene ID #:
4791
NCBI Official Name:
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
NCBI Official Symbol:
NFKB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
100 kDa
Protein Accession #:
NP_001070962.1
Protein GI Number:
117320527
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of NF-?B p100 only when phosphorylated at serine 872.
Swissprot #:
Q00653