ProSci

ALK (phospho Tyr1507) Antibody

Product Code:
 
PSI-80-015
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20°C for up to one year.
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Western blot analysis of extracts from COS7 cells treated with Anisomycin using Phospho-ALK (Tyr1507) Antibody. The lane on the right is treated with the antigen-specific peptide.
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Western blot analysis of extracts from COS7 cells treated with Anisomycin using Phospho-ALK (Tyr1507) Antibody. The lane on the right is treated with the antigen-specific peptide.

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PSI-80-015-0.1mg0.1mg£488.00
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Further Information

Additional Names:
kinase ALK, Anaplastic lymphoma kinase, CD246, ALK tyrosine kinase receptor precursor,
Application Note:
Western Blot: 1:500~1:1000
Background:
Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Background References:
  • Degoutin J, Vigny M, Gouzi JY (2007) FEBS Lett 581, 727-34.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ALK (phospho Tyr1507) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 1507 (P-T-Y (p)-G-S) derived from Human ALK.
Modifications:
Phospho-Specific
NCBI Gene ID #:
238
NCBI Official Name:
anaplastic lymphoma receptor tyrosine kinase
NCBI Official Symbol:
Alk
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
176 kDa
Protein Accession #:
NP_004295.2
Protein GI Number:
110347475
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific
SPECIFICITY:
The antibody detects endogenous level of ALK only when phosphorylated at tyrosine 1507.
Swissprot #:
Q9UM73