Further Information
Sphingosine 1-phosphate receptor 2, S1P2, AGR16, EDG-5, EDG5, Gpcr13, H218, LPB2
S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
- MacLennan AJ, Browe CS, Gaskin AA, et al. Cloning and characterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell Neurosci. 1994; 5:201-9.
- Van Brocklyn JR, Tu Z, Edsall LC, et al. Sphingosine 1-phosphate-induced cell rounding and neurite retraction are mediated by the G protein-coupled receptor H218. J. Biol. Chem. 1999; 274:4626-32.
- An S, Zheng Y, and Bleu T. Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5. J. Biol. Chem. 275:288-96.
- Zhang G, Yang L, Kim GS, et al. Critical role of sphingosine-1-phosphate receptor 2 (S1PR2) in acute vascular inflammation. Blood 2013; 122:443-55.
S1PR2 antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2.
The immunogen is located within amino acids 270 - 320 of S1PR2.
Molecular Weight Explanation:
multiple transmembrane protein, also multiple other S1PR2 antibodies also show a ~50kD band
9294
sphingosine-1-phosphate receptor 2
S1PR2
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39 kDa
Observed: 50 kDa
NP_004221
134244587
S1PR2 antibody is affinity chromatography purified via peptide column.
Stem Cell,Signal Transduction,Immunology
S1PR2 antibody is human, mouse and rat reactive.
O95136
Optimal dilutions for each application to be determined by the researcher.