Further Information
Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5
LRRC8A antibody can be used for detection of LRRC8A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).
- Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13.
- Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52.
- Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.
LRRC8A antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A.
The immunogen is located within amino acids 60 - 110 of LRRC8A.
Molecular Weight Explanation:
multiple other LRRC8A antibodies also show a ~95kD band
56262
leucine rich repeat containing 8 family, member A
LRRC8A
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 89 kDa
Observed: 95 kDa
NP_062540
62241040
LRRC8A antibody is affinity chromatography purified via peptide column.
Stem Cell,Signal Transduction
LRRC8A antibody is human, mouse and rat reactive.
Q8IWT6
Optimal dilutions for each application to be determined by the researcher.