Further Information
TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
- Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
- Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61.
- Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41.
- Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3.
The immunogen is located within amino acids 710 - 760 of TRPC3.
7222
transient receptor potential cation channel, subfamily C, member 3
TRPC3
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 90, 93, 101 kDa
Observed: 93 kDa
NP_001124170
194733735
TRPC3 Antibody is affinity chromatography purified via peptide column.
Signal Transduction,Neuroscience
TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.
Q13507
Optimal dilutions for each application to be determined by the researcher.