Further Information
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
This recombinant protein can be used for WB. For research use only.
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
- Dong H., et al., 1999, Nat. Med. 5:1365-1369.
- Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
- Nishimura H., et al., 2001, Trends?in?Immunology?22:265.?
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1 /B7-H1 /CD274 Protein, C-Fc Tag at 5μg/mL (100 μl/well),can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged. The EC50 is typically 16. 4-196. 8 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1 /B7-H1 /CD274 Protein, C-Fc Tag at 5μg/mL (100 μl/well),can bind Biotinylated Human PDCD1 Protein,C-Fc Tag. The EC50 is typically 25-227 ng/mL.
50 mM tris, 100 mM glycine, pH7.5
C-Fc Tag
29126
CD274 molecule
CD274
Homo sapiens
Lyophilized
Predicted Molecular Weight:
51.4 kDa
NP_054862.1
>95% as determined by SDS-PAGE and SEC-HPLC.
Recombinant Protein Sequence:
Thr32-Gly402
HEK293 cells
Q9NZQ7