ProSci

GLP1R Antibody

Product Code:
 
PSI-43-439
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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43-439 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-439 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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43-439 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-439 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

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PSI-43-439-0.1mg0.1mg£626.00
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Further Information

Additional Names:
Glp1r, glucagon-like peptide 1 receptor, GLP-1R, GLP1Rc, GLP-1 receptor, GLP-1-R
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Hippocampus and in Rat and Pig Brain lysates (calculated MW of 53.0kDa according to Human NP_002053.3, 52.9kDa according to Rat NP_036860.1 and 52.7kDa according to Pig NP_001243523.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Lamont BJ, Li Y, Kwan E, Brown TJ, Gaisano H, Drucker DJ.Pancreatic GLP-1 receptor activation is sufficient for incretin control of glucose metabolism in mice.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QHQWDGLLSYQD
NCBI Gene ID #:
2740
NCBI Official Name:
glucagon like peptide 1 receptor
NCBI Official Symbol:
GLP1R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 55kDa
Protein Accession #:
NP_002053.3
Protein GI Number:
166795283
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,
SPECIFICITY:
The immunizing peptide represents part of an extracellular region.
Swissprot #:
P43220