ProSci

Thrombospondin Antibody

Product Code:
 
PSI-43-470
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human THBS1 with C-terminal MYC tag probed with 43-470 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-470 (1ug/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human THBS1 with C-terminal MYC tag probed with 43-470 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-470 (1ug/ml) in Lane B. Primary

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PSI-43-470-0.1mg0.1mg£626.00
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Further Information

Additional Names:
thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:In transfected HEK293 transiently expressing Human THBS1 a band of approx. 200kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 129kDa according to NP_003237.2. Recommended concentration, 1-3ug/ml.
Background References:
  • Samols MA, Skalsky RL, Maldonado AM, Riva A, Lopez MC, Baker HV, Renne R.Identification of ular genes targeted by KSHV-encoded microRNAs.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: EGEARETKACKKD
NCBI Gene ID #:
7057
NCBI Official Name:
thrombospondin 1
NCBI Official Symbol:
THBS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003237.2
Protein GI Number:
40317626
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
Swissprot #:
P07996