ProSci

Fgf14 Antibody

Product Code:
 
PSI-43-179
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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43-179 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-179 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-43-179-0.1mg0.1mg£626.00
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Further Information

Additional Names:
Fgf14, fibroblast growth factor 14, FHF-4, Fhf4, mFHF-4(1B), FGF-14, fibroblast growth factor homologous factor 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.
Background References:
  • Shakkottai VG, Xiao M, Xu L, Wong M, Nerbonne JM, Ornitz DM, Yamada KA.FGF14 regulates the intrinsic excitability of cerebellar Purkinje neurons.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: REQHWDRPSASR-C
NCBI Gene ID #:
2259
NCBI Official Name:
fibroblast growth factor 14
NCBI Official Symbol:
Fgf14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 28kDa
Protein Accession #:
NP_034331.2, NP_997550.1
Protein GI Number:
46575905
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
SPECIFICITY:
This antibody is expected to recognize mouse isoform a (NP_034331.2) and human isoform 1A (NP_004106.1).
Swissprot #:
Q92915