Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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United States.
Typical lead time:
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Further Information
ACTN1, actinin, alpha 1, F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, alpha-actinin-1, non-muscle alpha-actinin-1
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human, Mouse and Rat Spleen lysates (calculated MW of 103kDa according to NP_001093.1). Recommended concentration: 0.3-1ug/ml.
An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- Quick Q, Skalli O.Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
The immunogen for this antibody is: C-TPQEINGKWDHVRQ
87
actinin, alpha 1
ACTN1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 100kDa
NP_001123476.1, NP_001093.1, NP_001123477.1
4501891
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer,Cancer
This antibody is expected to recognize all reported isoforms (NP_001123476.1; NP_001093.1; NP_001123477.1).
P12814