Chemodex

DCFDA

Product Code:
 
CDX-D0063
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
+20°C
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Chemical Structure
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Chemical Structure

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CDX-D0063-G0011 g£78.00
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CDX-D0063-G0055 g£280.00
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Further Information

Alternate Names/Synonyms:
2',7'-Dichlorofluorescein diacetate; Diacetyldichlorofluorescein; DCFH-DA
Appearance:
White to off-white powder.
CAS:
2044-85-1
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
InChiKey:
VQVUBYASAICPFU-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 2044-85-1. Formula: C24H14Cl2O7. MW: 485.27. Synthetic. A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2?,7?-dichlorofluorescein (DCF). Spectral data: lambdaex=504nm, lambdaem=524nm in 0.1 M Tris pH 8.0 (esterase).
MDL:
MFCD00037501
Molecular Formula:
C24H14Cl2O7
Molecular Weight:
485.27
Package Type:
Vial
Product Description:
A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2?,7?-dichlorofluorescein (DCF). Spectral data: lambdaex=504nm, lambdaem=524nm in 0.1 M Tris pH 8.0 (esterase).
Purity:
>95% (HPLC)
SMILES:
CC(=O)OC1=C(Cl)C=C2C(OC3=CC(OC(C)=O)=C(Cl)C=C3C22OC(=O)C3=C2C=CC=C3)=C1
Solubility Chemicals:
Soluble in DMSO or acetone.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) A.R. Rosenkranz, et al.; J. Immunol. Meth. 156, 39 (1992) | (2) T. Takanashi, et al.; Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997) | (3) E. Eruslanov & S. Kusmartsev; Methods Mol. Biol. 594, 57 (2010)