ProSci

Nampt Antibody [OMNI379]

Product Code:
 
PSI-36-034
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
OMNI379
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Stable for at least 1 year after receipt when stored at -20˚C.
 

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PSI-36-034-100ug100ug£657.00
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Further Information

Additional Names:
Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Application Note:
ELISA: (direct or indirect: 1:2,000-1:10,000). Flow Cytometry: (1:1,000). Immunocytochemistry: (1:500-1:1,000). Immunohistochemistry: (paraffin sections, 1:100-1:500). Immunoprecipitation: (1:500-1:1,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Background:
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human Nampt (visfatin/PBEF).
NCBI Gene ID #:
10135
NCBI Official Name:
nicotinamide phosphoribosyltransferase
NCBI Official Symbol:
NAMPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
5031977
Protein GI Number:
NP_005737
Research Area:
Cancer,Obesity
SPECIFICITY:
Recognizes human, mouse and rat Nampt.
Swissprot #:
P43490
User NOte:
Optimal dilutions for each application to be determined by the researcher.