ProSci

NAD Kinase B. subtilis Recombinant Protein

Product Code:
 
PSI-90-364
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Shipping:
 
Dry ice
Storage:
 
Stable for at least 6 months after receipt when stored at -80˚C.
 

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PSI-90-364-50ug50ug£673.00
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Further Information

Additional Names:
Probable Inorganic Polyphosphate/ATP-NAD Kinase, Poly(P)/ATP NAD Kinase, ppnK, yjbN, EC 2.7.1.23
Application Note:
This recombinant proteins is for research use only.
Background:
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+
Biological Activity:
~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min.
Buffer:
In 50mM TRIS-HCl, pH 7.0 containing 150mM sodium chloride, 1mM EDTA and 1mM dithiothreitol.
Fusion Tag:
Tag Free
NCBI Gene ID #:
936414
NCBI Official Name:
inorganic polyphosphate/ATP-NAD kinase 1
NCBI Official Symbol:
ppnK
NCBI Organism:
Bacillus subtilis subsp. subtilis str. 168
Physical State:
Liquid
Protein Accession #:
O31612
Protein GI Number:
8928487
Purity:
>98% (SDS-PAGE)
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q07011

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