ProSci

NEDD8 Recombinant Protein

Product Code:
 
PSI-92-177
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Storage:
 
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
 

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PSI-92-177-0.05mg0.05mg£373.00
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Further Information

Additional Names:
Neural precursor cell expressed developmentally down-regulated protein 8, NEDD8, Neddylin, Ubiquitin-like protein Nedd8
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
N-6 His, SUMO tag
NCBI Gene ID #:
4738
NCBI Official Name:
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol:
NEDD8
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
20.9 kD
Protein Accession #:
Q15843
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q15843

Documents