ProSci

Tryptophan synthase Recombinant Protein

Product Code:
 
PSI-92-667
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Escherichia coli
Storage:
 
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
 

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PSI-92-667-0.05mg0.05mg£657.00
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Further Information

Additional Names:
Tryptophan synthetase, Tryptophan synthase
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Tryptophan synthase is a multienzyme alpha2 beta 2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The alpha-subunit catalyzes cleavage of 3-indole-d-glycerol 3'-phosphate (IGP) to give indole and D-glyceraldehyde 3'-phosphate (G3P). Indole is then transferred through a 25-? hydrophobic tunnel to the beta -subunit. The beta 2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.
Buffer:
Lyophilized from a 0.2 um filtered solution of PBS, pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
N-6 His tag
NCBI Gene ID #:
946204
NCBI Official Name:
tryptophan synthase, alpha subunit
NCBI Official Symbol:
trpA
NCBI Organism:
Escherichia coli str. K-12 substr. MG1655
Physical State:
Lyophilized
Predicted Molecular Weight:
72.5 kD
Protein Accession #:
P0A877, P0A879
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P0A877, P0A879

Documents