ProSci

PTN Antibody

Product Code:
 
PSI-63-201
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
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PTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
PTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-63-201-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Heparin-binding growth-associated molecule, HB-GAM, Heparin-binding neurite outgrowth-promoting factor 1, HBNF-1, Osteoblast-specific factor 1, OSF-1, PTN, HBNF1, NEGF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Pleiotrophin or Neurite Growth-promoting Factor 1 (NEGF1), also called Heparin Affin Regulatory Peptide (HARP) or Heparin Binding Growth associated molecule (HB-GAM) is an 18-kDa growth factor that has a high affinity for heparin. It is structurally related to midkine and retinoic acid induced heparin-binding protein.
Background References:
  • Park,T.J., Mol. Carcinog. 47 (10), 784-796 (2008)
  • Li,J., J. Biol. Chem. 282 (48), 34984-34993 (2007)
  • Li,Y.S., J. Biol. Chem. 267 (36), 26011-26016 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This PTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human PTN.
NCBI Gene ID #:
5764
NCBI Official Name:
Pleiotrophin
NCBI Official Symbol:
PTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
P21246
Protein GI Number:
131553
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Neuroscience,Signal Transduction
Swissprot #:
P21246
User NOte:
Optimal dilutions for each application to be determined by the researcher.