ProSci

DIO1 Antibody

Product Code:
 
PSI-62-559
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution + human kidney whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of DIO1 Antibody in HepG2 cell line lysates (35ug/lane)
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Western Blot at 1:2000 dilution + human kidney whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of DIO1 Antibody in HepG2 cell line lysates (35ug/lane)

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PSI-62-559-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1
Application Note:
For WB starting dilution is: 1:1000
Background:
DIO1 is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD).
Background References:
  • Landa,I., et.al., PLoS Genet.(9), E1000637 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DIO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-57 amino acids from the N-terminal region of human DIO1.
NCBI Gene ID #:
1733
NCBI Official Name:
Type I iodothyronine deiodinase
NCBI Official Symbol:
DIO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P49895
Protein GI Number:
182702125
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P49895
User NOte:
Optimal dilutions for each application to be determined by the researcher.