ProSci

PLA2G7 Antibody

Product Code:
 
PSI-64-131
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 4
Overlay histogram showing HL-60 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 4
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: human lung lysate Lane 3: human spleen lysate Lane 4: human plasma lysate Lysates/proteins at 20 ug per lane.
3 / 4
4 / 4
PLA2G7 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.
❮❯

Overlay histogram showing HL-60 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: human lung lysate Lane 3: human spleen lysate Lane 4: human plasma lysate Lysates/proteins at 20 ug per lane.
PLA2G7 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-64-131-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, Group-VIIA phospholipase A2, gVIIA-PLA2, LDL-associated phospholipase A2, LDL-PLA(2), PAF 2-acylhydrolase, PLA2G7, PAFAH
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency.
Background References:
  • Fan, P., et al. Hum. Reprod. 25(5):1288-1294(2010)
  • Paik, J.K., et al. Clin. Chim. Acta 411 (7-8), 486-493 (2010)
  • Meng, X., et al. Psychiatry Res 175 (1-2), 186-187 (2010)
  • McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PLA2G7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human PLA2G7.
NCBI Gene ID #:
7941
NCBI Official Name:
Platelet-activating factor acetylhydrolase
NCBI Official Symbol:
PLA2G7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q13093
Protein GI Number:
2497687
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q13093
User NOte:
Optimal dilutions for each application to be determined by the researcher.