ProSci

NCS1 Antibody

Product Code:
 
PSI-58-187
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of NCS1 Antibody in MCF-7 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of NCS1 Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-58-187-400ul400ul£626.00
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Further Information

Additional Names:
Neuronal calcium sensor 1, NCS-1, Frequenin homolog, Frequenin-like protein, Frequenin-like ubiquitous protein, NCS1, FLUP, FREQ
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. This protein is thought to be associated with secretory granules and may be involved in the regulation of neurosecretion.
Background References:
  • Koh, P.O., et al., Proc. Natl. Acad. Sci. U.S.A. 100(1):313-317 (2003).
  • Bourne, Y., et al., J. Biol. Chem. 276(15):11949-11955 (2001).
  • Burgoyne, R.D., et al., Biochem. J. 353 (Pt 1), 1-12 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat, Xenopus
Immunogen:
This NCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NCS1.
NCBI Gene ID #:
23413
NCBI Official Name:
Neuronal calcium sensor 1
NCBI Official Symbol:
NCS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
P62166
Protein GI Number:
49065666
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P62166
User NOte:
Optimal dilutions for each application to be determined by the researcher.