ProSci

PPP2R2A Antibody

Product Code:
 
PSI-61-002
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 4
Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 4
Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
3 / 4
4 / 4
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

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PSI-61-002-400ul400ul£626.00
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Further Information

Additional Names:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, PP2A subunit B isoform B55-alpha, PP2A subunit B isoform PR55-alpha, PP2A subunit B isoform R2-alpha, PP2A subunit B isoform alpha, PPP2R2A
Application Note:
For IHC-P starting dilution is: 1:25

For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Background References:
  • Mayer R.E., et al. Biochemistry 30:3589-3597(1991).
  • Ota T., et al. Nat. Genet. 36:40-45(2004).
  • Nusbaum C., et al. Nature 439:331-335(2006).
  • Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse, Pig, Rabbit, Rat
Immunogen:
This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
NCBI Gene ID #:
5520
NCBI Official Name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
NCBI Official Symbol:
PPP2R2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P63151
Protein GI Number:
52783535
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P63151
User NOte:
Optimal dilutions for each application to be determined by the researcher.