ProSci

LAP3 Antibody

Product Code:
 
PSI-62-802
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-LAP3Pab in HepG2 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LAP3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-LAP3Pab in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LAP3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-802-400ul400ul£626.00
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Further Information

Additional Names:
Cytosol aminopeptidase, Leucine aminopeptidase 3, LAP-3, Leucyl aminopeptidase, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase, LAP3, LAPEP, PEPS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
LAP3 is presumably involved in the processing and regular turnover of intracellular proteins. It catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro.
Background References:
  • Goto,Y.,FEBS Lett. 580 (7), 1833-1838 (2006)
  • Matsushima,M.,Biochem. Biophys. Res. Commun. 178 (3), 1459-1464 (1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human LAP3.
NCBI Gene ID #:
51056
NCBI Official Name:
Cytosol aminopeptidase
NCBI Official Symbol:
LAP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
P28838
Protein GI Number:
124028615
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P28838
User NOte:
Optimal dilutions for each application to be determined by the researcher.