ProSci

CAF-1 Antibody

Product Code:
 
PSI-55-638
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:1000 dilution + HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
CAF-1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western Blot at 1:1000 dilution + HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.
CAF-1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-638-400ul400ul£626.00
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Further Information

Additional Names:
CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, CAF-1, Caf1a, CNOT7, CAF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Background References:
  • Lau, N.C., et al. Biochem. J. 422(3):443-453(2009)
  • Aslam, A., et al. Mol. Biol. Cell 20(17):3840-3850(2009)
  • Miyasaka, T., et al. Cancer Sci. 99(4):755-761(2008)
  • Nishida, K., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 63 (PT 12), 1061-1063 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Mouse, Xenopus
Immunogen:
This CAF-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-61 amino acids from the N-terminal region of human CAF-1.
NCBI Gene ID #:
29883
NCBI Official Name:
CCR4-NOT transcription complex subunit 7
NCBI Official Symbol:
CNOT7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q9UIV1
Protein GI Number:
41713629
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UIV1
User NOte:
Optimal dilutions for each application to be determined by the researcher.