ProSci

EIF5 Antibody

Product Code:
 
PSI-61-280
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-61-280-200ul200ul£626.00
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Further Information

Additional Names:
Eukaryotic translation initiation factor 5, eIF-5, EIF5
Application Note:
For WB starting dilution is: 1:2000
Background:
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Background References:
  • Si K.,et al.J. Biol. Chem. 271:16934-16938(1996).
  • Wiemann S.,et al.Genome Res. 11:422-435(2001).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EIF5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 267-298 amino acids from the Central region of human EIF5.
NCBI Gene ID #:
1983
NCBI Official Name:
Eukaryotic translation initiation factor 5
NCBI Official Symbol:
EIF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P55010
Protein GI Number:
27735202
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P55010
User NOte:
Optimal dilutions for each application to be determined by the researcher.