ProSci

POLD1 Antibody

Product Code:
 
PSI-61-563
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of POLD1 Antibody in Jurkat cell line lysates (35ug/lane)
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Flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of POLD1 Antibody in Jurkat cell line lysates (35ug/lane)
Flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-563-400ul400ul£626.00
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Further Information

Additional Names:
DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, POLD1, POLD
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.
Background References:
  • Chung D.W., Zhang J.,Proc. Natl. Acad. Sci. U.S.A. 88:11197-11201(1991)
  • Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992)
  • Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This POLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 710-739 amino acids from the Central region of human POLD1.
NCBI Gene ID #:
5424
NCBI Official Name:
DNA polymerase delta catalytic subunit
NCBI Official Symbol:
POLD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
124 kDa
Protein Accession #:
P28340
Protein GI Number:
50403732
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P28340
User NOte:
Optimal dilutions for each application to be determined by the researcher.