ProSci

GTF2E1 Antibody

Product Code:
 
PSI-57-730
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in 293 cell line lysates (35ug/lane).
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GTF2E1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in 293 cell line lysates (35ug/lane).
GTF2E1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-57-730-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
General transcription factor IIE subunit 1, General transcription factor IIE 56 kDa subunit, Transcription initiation factor IIE subunit alpha, TFIIE-alpha, GTF2E1, TF2E1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Background References:
  • Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
  • Okuda, M., et al. EMBO J. 27(7):1161-1171(2008)
  • Di Lello, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):106-111(2008)
  • Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GTF2E1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-121 amino acids from the N-terminal region of human GTF2E1.
NCBI Gene ID #:
2960
NCBI Official Name:
General transcription factor IIE subunit 1
NCBI Official Symbol:
GTF2E1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P29083
Protein GI Number:
116242812
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P29083
User NOte:
Optimal dilutions for each application to be determined by the researcher.