ProSci

ACF Antibody

Product Code:
 
PSI-55-326
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in A375 cell line lysates (35ug/lane).
2 / 3
ACF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in A375 cell line lysates (35ug/lane).
ACF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-326-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312|EMBL:CAB947541}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.
Background References:
  • Galloway, C.A., et al. Biochem. Biophys. Res. Commun. 391(1):659-663(2010)
  • Blanc, V., et al. Mol. Cell. Biol. 25(16):7260-7269(2005)
  • Deloukas, P., et al. Nature 429(6990):375-381(2004)
  • Xie, K., et al. Proc. Natl. Acad. Sci. U.S.A. 101(21):8114-8119(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.
NCBI Gene ID #:
29974
NCBI Official Name:
APOBEC1 complementation factor
NCBI Official Symbol:
A1CF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q9NQ94
Protein GI Number:
74761651
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NQ94
User NOte:
Optimal dilutions for each application to be determined by the researcher.