ProSci

HNRPL Antibody

Product Code:
 
PSI-61-621
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of HNRPL Antibody in HepG2, Jurkat cell line lysates (35ug/lane)
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Western blot analysis of HNRPL using rabbit polyclonal HNRPL Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HNRPL gene (Lane 2).
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of HNRPL Antibody in HepG2, Jurkat cell line lysates (35ug/lane)
Western blot analysis of HNRPL using rabbit polyclonal HNRPL Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HNRPL gene (Lane 2).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-621-400ul400ul£626.00
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Further Information

Additional Names:
Heterogeneous nuclear ribonucleoprotein L, hnRNP L, HNRNPL, HNRPL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
HNRPL is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. L is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes.
Background References:
  • Hahm,B., et.al., FEBS Lett. 425 (3), 401-406 (1998)
  • Funke,B., et.al., Nucleic Acids Res. 24 (19), 3821-3828 (1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This HNRPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human HNRPL.
NCBI Gene ID #:
3191
NCBI Official Name:
Heterogeneous nuclear ribonucleoprotein L
NCBI Official Symbol:
HNRNPL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
P14866
Protein GI Number:
215274006
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P14866
User NOte:
Optimal dilutions for each application to be determined by the researcher.