ProSci

CPN1 Antibody

Product Code:
 
PSI-62-834
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of CPN1 antibody in K562, CEM, HEpG2 cell line lysates and mouse liver tissue lysates (35ug/lane)
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Western Blot at 1:1000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CPN1 antibody in K562, CEM, HEpG2 cell line lysates and mouse liver tissue lysates (35ug/lane)

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PSI-62-834-400ul400ul£626.00
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Further Information

Additional Names:
Carboxypeptidase N catalytic chain, CPN, Anaphylatoxin inactivator, Arginine carboxypeptidase, Carboxypeptidase N polypeptide 1, Carboxypeptidase N small subunit, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, Serum carboxypeptidase N, SCPN, CPN1, ACBP
Application Note:
For WB starting dilution is: 1:1000
Background:
CPN1 is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The protein is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This protein is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits.
Background References:
  • Davis,D.A., Singer,K.E. Blood 105 (12), 4561-4568 (2005)
  • Riley,D.A., Tan,F. Genomics 50 (1), 105-108 (1998)
  • Hendriks,D., Vingron,M. Biol. Chem. Hoppe-Seyler 374 (9), 843-849 (1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the N-terminal region of human CPN1.
NCBI Gene ID #:
1369
NCBI Official Name:
Carboxypeptidase N catalytic chain
NCBI Official Symbol:
CPN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P15169
Protein GI Number:
115896
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
P15169
User NOte:
Optimal dilutions for each application to be determined by the researcher.