ProSci

DNAJC6 Antibody

Product Code:
 
PSI-64-222
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).
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DNAJC6 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).
DNAJC6 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-222-400ul400ul£626.00
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Further Information

Additional Names:
Putative tyrosine-protein phosphatase auxilin, DnaJ homolog subfamily C member 6, DNAJC6, KIAA0473
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain
Background References:
  • Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
  • Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
  • Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
  • Hirst, J., et al. Traffic 9(8):1354-1371(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6.
NCBI Gene ID #:
9829
NCBI Official Name:
Putative tyrosine-protein phosphatase auxilin
NCBI Official Symbol:
DNAJC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
100 kDa
Protein Accession #:
O75061
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O75061
User NOte:
Optimal dilutions for each application to be determined by the researcher.