ProSci

Reptin/TIP49B/RUVB2 Antibody

Product Code:
 
PSI-60-401
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Antibody is used in Western blot to detect RUVBL2 in mouse kidney (left) and SK-Br-3 (right) tissue/cell line lysates.
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Western blot analysis of RUVBL2 using rabbit polyclonal RUVBL2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL2 gene.
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Antibody is used in Western blot to detect RUVBL2 in mouse kidney (left) and SK-Br-3 (right) tissue/cell line lysates.
Western blot analysis of RUVBL2 using rabbit polyclonal RUVBL2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL2 gene.

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PSI-60-401-400ul400ul£626.00
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Further Information

Additional Names:
RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, 51 kDa erythrocyte cytosolic protein, ECP-51, INO80 complex subunit J, Repressing pontin 52, Reptin 52, TIP49b, TIP60-associated protein 54-beta, TAP54-beta, RUVBL2, INO80J, TIP48, TIP49B
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
Background References:
  • Bauer, A., et al., EMBO J. 19(22):6121-6130 (2000).
  • Parfait, B., et al., Ann. Genet. 43(2):69-74 (2000).
  • Wood, M.A., et al., Mol. Cell 5(2):321-330 (2000).
  • Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine
Immunogen:
This Reptin/TIP49B/RUVB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-430 amino acids from the C-terminal region of human Reptin/TIP49B/RUVB2.
NCBI Gene ID #:
10856
NCBI Official Name:
RuvB-like 2
NCBI Official Symbol:
RUVBL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q9Y230
Protein GI Number:
28201890
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9Y230
User NOte:
Optimal dilutions for each application to be determined by the researcher.