ProSci

Mouse Cdkn2a Antibody

Product Code:
 
PSI-60-398
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 1
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
❮❯

Western blot analysis in mouse spleen tissue lysates (35ug/lane).

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-60-398-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Cyclin-dependent kinase inhibitor 2A, isoform 3, p19ARF, Cdkn2a {ECO:0000312|EMBL:AAB357701, ECO:0000312|MGI:MGI:104738}
Application Note:
For WB starting dilution is: 1:1000
Background:
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.
Background References:
  • Nogueira, C., et al. Oncogene 29(47):6222-6232(2010)
  • Fernandez-Diaz, L.C., et al. Development 137(20):3393-3403(2010)
  • Bennecke, M., et al. Cancer Cell 18(2):135-146(2010)
  • Negishi, M., et al. PLoS ONE(8), E12373 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Cdkn2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-65 amino acids from the Central region of mouse Cdkn2a.
NCBI Gene ID #:
12578
NCBI Official Name:
Cyclin-dependent kinase inhibitor 2A, isoform 3
NCBI Official Symbol:
Cdkn2a
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q64364
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q64364
User NOte:
Optimal dilutions for each application to be determined by the researcher.