ProSci

Arhgef9 Antibody

Product Code:
 
PSI-61-443
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-443-400ul400ul£626.00
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Further Information

Additional Names:
Rho guanine nucleotide exchange factor 9, Collybistin, Rac/Cdc42 guanine nucleotide exchange factor 9, Arhgef9
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
ARHGEF9 belongs to a family of Rho-like GTPases that act as molecular switches by cycling from the active GTP-bound state to the inactive GDP-bound state. These proteins are key regulators of the actin cytoskeleton and are involved in cell signaling.
Background References:
  • Xiang,S., J. Mol. Biol. 359 (1), 35-46 (2006)
  • Harvey,K., J. Neurosci. 24 (25), 5816-5826 (2004)
  • Grosskreutz,Y., Biol. Chem. 382 (10), 1455-1462 (2001)
  • Kins,S., Nat. Neurosci.(1), 22-29 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This Arhgef9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human Arhgef9.
NCBI Gene ID #:
66013
NCBI Official Name:
Rho guanine nucleotide exchange factor 9
NCBI Official Symbol:
Arhgef9
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
Q9QX73
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9QX73
User NOte:
Optimal dilutions for each application to be determined by the researcher.