South Bay Bio

NEDD8 (human) (rec.) (Rhodamine 110)

Product Code:
 
SBB-PS0003
Product Group:
 
Recombinant Proteins
Supplier:
 
South Bay Bio
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Dry Ice
Storage:
 
-80C
1 / 1
Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
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Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.

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SBB-PS0003-C05050 ug£415.00
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Further Information

Alternate Names/Synonyms:
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM MES pH 6.0, 150mM sodium chloride.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.Protect from light.
Labels - Conjugates:
Rhodamine
Long Description:
Protein. Human NEDD8 (aa1-76)conjugated at the C-terminus to a quenched Rhodamine 110 dye. Source: E. coli. Formulation: Liquid. In 50mM MES pH 6.0, 150mM sodium chloride. Purity: >97% (LCMS). NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle.NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the C-terminus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rhodamine 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1.
Molecular Weight:
~9kDa
NCBI, Uniprot Number:
Q15843
Package Type:
Plastic Vial
Product Description:
NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the C-terminus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rhodamine 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1.
Purity:
>97% (LCMS)
Sequence:
Human NEDD8 (aa1-76) (Accession Nr. Q15843 http://www.ncbi.nlm.nih.gov/protein/Q15843 ) conjugated at the C-terminus to a quenched Rhodamine 110 dye.
Source / Host:
E. coli
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.