ProSci

VISTA Detection Set (Risk Free)

Product Code:
 
PSI-RF16070
Product Group:
 
Antibody Detection Sets
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
See Set Contents
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Mouse Monoclonal: Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year.

As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 

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PSI-RF16070-1Set1Set£337.00
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  • Further Information
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Further Information

Background:
VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation. ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
Background References:
  • Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
  • Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Conjugate:
Unconjugated
Immunogen:
VISTA antibodies were raised against the extracellular domain of human VISTA.
Physical State:
Liquid
Purification:
Antibodies are supplied as protein A purified IgG1 and IgG2b.
Set Contents:
VISTA Antibody [4C4], Cat. No. RF16071 (20 μg)

VISTA Antibody [6D2], Cat. No. RF16072 (20 μg)
VISTA Antibody [8E11], Cat. No. RF16073 (20 μg)
VISTA Antibody [9E4], Cat. No. RF16074 (20 μg)
User Note:
Optimal dilutions for each application to be determined by the researcher.

Documents

References

  1. Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
  2. Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.