ProSci

TIGIT Recombinant Protein

Product Code:
 
PSI-96-955
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-955-0.1mg0.1mg£729.00
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Further Information

Additional Names:
TIGIT,VSIG9,VSTM3
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells.
Background References:
  • Chew GM., et al., 2016, PLoS Pathogens., 12: e1005349.
  • Joe-Marc Chauvin, et al., 2015, J Clin Invest., 125 (5): 2046-2058.
  • Steven G. Deeks, 2012, Nature, 487 (1): 439-440.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CD155, Fc Tag at 5 ug/mL (100 uL/well) can bind Human TIGIT, mouse IgG2a Fc tag with a linear range of 3.9-62.5 ng/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
mouse IgG2a Fc tag
NCBI Gene ID #:
201633
NCBI Official Name:
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol:
TIGIT
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
40.1 kDa
Protein Accession #:
AAI01290
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 0.1 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q495A1

Documents