ProSci

GAPDH Recombinant Protein

Product Code:
 
PSI-96-878
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-878-0.1mg0.1mg£597.00
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Further Information

Additional Names:
GAPDH,GAPD,CDABP0047,OK/SW-cl.12
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is also known as Peptidyl-cysteine S-nitrosylase GAPDH, which is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH can participate in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Also, GAPDH can modulate the organization and assembly of the cytoskeleton. Furthermore, GAPDH is a component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.
Background References:
  • Tokunaga K., et al., 1987, Cancer Res. 47:5616-5619.
  • Allen R.W., et al., 1987, J. Biol. Chem. 262:649-653.
  • Jarczowski F., et al., 2009, J. Biol. Chem. 284:766-773.
Buffer:
50 mM Tris, 150 mM NaCl, pH7.5
Fusion Tag:
His Tag
NCBI Gene ID #:
2597
NCBI Official Name:
Glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:
GAPDH
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
36.9 kDa
Protein Accession #:
AAH01601
Purity:
>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P04406

Documents