NSJ Bioreagents

Beta-Actin Antibody

Product Code:
 
NSJ-R30100
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AC-15
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the beta-Actin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of beta-Actin antibody and rat tissue: ovary (1), testis (2), heart (3), brain (4); and human cell lines, lanes 5: A453; 6: HeLa; 7: SMMC-7721; 8: HT1080; 9: SW620 cell lysate
2 / 7
Western blot testing of beta-Actin antibody and rat tissues: liver (1), spleen (2), brain (3), kidney (4) and human cell lines, lane 5: HeLa; 6: SMMC-7721; 7: SGC cell lysate
3 / 7
IHC testing of FFPE human lung cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
4 / 7
IHC testing of FFPE human intestinal cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
5 / 7
IHC testing of FFPE human breast cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
6 / 7
IHC testing of FFPE mouse intestine with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
7 / 7
IHC testing of FFPE rat intestine with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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Western blot testing of beta-Actin antibody and rat tissue: ovary (1), testis (2), heart (3), brain (4); and human cell lines, lanes 5: A453; 6: HeLa; 7: SMMC-7721; 8: HT1080; 9: SW620 cell lysate
Western blot testing of beta-Actin antibody and rat tissues: liver (1), spleen (2), brain (3), kidney (4) and human cell lines, lane 5: HeLa; 6: SMMC-7721; 7: SGC cell lysate
IHC testing of FFPE human lung cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human intestinal cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse intestine with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat intestine with beta-Actin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

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CodeSizePrice
NSJ-R30100-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of the protein that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
60
Immunogen:
Amino acids DDDIAALVIDNGSGK were used as the immunogen for this beta-Actin antibody.
Limitation:
This beta-Actin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, mouse, rat