NSJ Bioreagents

Chk2 Antibody / CHEK2

Product Code:
 
NSJ-R31854
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Chk2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
Western blot testing of 1) rat spleen, 2) mouse testis, 3) human SW620 lysate with Chk2 antibody. Expected molecular weight: ~61 kDa.~
2 / 2
IHC testing of FFPE human lung cancer tissue with Chk2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM
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Western blot testing of 1) rat spleen, 2) mouse testis, 3) human SW620 lysate with Chk2 antibody. Expected molecular weight: ~61 kDa.~
IHC testing of FFPE human lung cancer tissue with Chk2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM

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NSJ-R31854-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the Chk2 antibody should be determined by the researcher.
Description:
Chk2 is a serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. [UniProt]
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDL of human Chk2/CHEK2 were used as the immunogen for the Chk2 antibody.
Limitation:
This Chk2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O96017